新手在膜片钳电生理实验中，往往被封接与破膜这两道关卡困扰，如同置身于迷雾重重的森林，不知所措。

封接阶段就像是一场微观的舞蹈，需要微妙的平衡。新手往往在寻找和建立液-液界面时感到困惑，电极与细胞膜的接触，既要接近，又不能过于猛烈，否则会损伤细胞，破坏实验的完整性。

主要功能

ez-gSEAL破膜仪可由iPod Touch，iPhone或iPAD控制。 iPod Touch通过WiFi连接到服务器软件NBSController，因此您可以使用iPod Touch作为遥控器来控制设备的设备。

Figure 1 Recording obtained at the Marine Biological Laboratory using the ez-gSEAL 100A pressure controller. LNV clock neurons in female Drosophila were used for electrophysiological recordings. A 6-7 micrometer diameter pipette filled with protease XIV was first used to digest the tissue above the clock neurons. The pressure setting for applying the protease XIV was 20 - 30 mmHg. When making the seal, 40 mmHg pressure was applied during the time the patch pipette (polished. Resistance ~ 17 MOhms) approached the neuron, and then -20 mmHg suction was applied to make the seal. The breaking-in pressure for this cell was -200 to -250 mmHg for 0.5 s. The holding pressure was 1 mmHg to keep the seal stable during the recording. This recording was obtained 3.5 hours after making the seal, and the cell was still healthy.   Faculties: Dr. Guan Cao, University of Texas; Dr. Michael Nitaback, Yale University.  

Figure 2 Suspension of HEK cells expressing ClC-0/C212S chloride channels. When making the seal, 5 mmHg pressure was applied during the time the patch pipette (Resistance ~ 2 MOhms) approached the cell, and then -40 mmHg suction was applied to make the seal. 
Obtained from Dr. Xiaodong Zhang and Dr. Tsung-Yu Chen, University of California, Davis. 

*后，给大家推荐几组软件设置参数，供大家参考。